Development of an Artificial Placenta: Endocrine Responses of Goat Fetuses during Long-Term Extrauterine Incubation with Umbilical Arteriovenous Extracorporeal Membrane Oxygenation

Endocrine Journal

KAZUNORI BABA³  MASATO SAKAI¹  SHIRO KOZUMA¹  TAKASHI OKAI¹  KIMIHIRO TAKECHI¹  HARUO MASUDA¹  YUMIKO NARUMIYA-TAKIKAWA¹  YOSHIKO OGAMI¹  YUJI TAKETANI¹  NOBUYA UNNO¹  RURIKO TSUSHIMA¹  YOSHINORI KUWABARA² 

[1] Laboratory of Perinatal Medicine, Department of Obstetrics and Gynecology, Faculty of Medicine, The University of Tokyo;Department of Obstetrics and Gynecology, Faculty of Medicine, Juntendo University;Institute of Medical Electronics, Faculty of Medicine, The University of Tokyo

关键词: Catecholamines;    Adrenocorticotropic hormone;    Cortisol;    Renal dysfunction;    Extrauterine fetal incubation;   

DOI  :  10.1507/endocrj.41.Supplement_S69

学科分类：内分泌与代谢学

来源: Japan Endocrine Society

【 摘 要 】

References(20)Cited-By(3)To investigate endocrine responses of isolated premature goat fetuses during long-term extrauterine incubation with umbilical arteriovenous extracorporeal membrane oxygenation (A-V ECMO), we conducted experiments in seven goat fetuses (95-134 days gestation). The fetuses were cannulated from the umbilical vessels, and their blood-gas exchange was totally supported by A-V ECMO, while they were maintained in an isothermal incubator containing artificial amniotic fluid. The survival period was between 84 and 190 h. At 24-h intervals, fetal blood samples were collected, and plasma concentrations of catecholamines, ACTH, and cortisol were determined. After 24 h of incubation, fetal circulatory and respiratory variables remained stable, until evident circulatory failure occurred before their deaths. A similar pattern was observed in temporal changes in plasma concentrations of catecholamines, ACTH, and cortisol. Plasma levels were high during the initial 24 h of incubation, subsequently decreased, and then increased before death. Hormone levels during stable periods were equivalent to or slightly higher than values for fetuses in utero. These results suggest that conditions during the stable period of long-term extracorporeal fetal incubation are not highly stressful for the isolated fetuses.

【 授权许可】

Unknown