Segment 8 encodes a structural protein ofinfectious salmon anaemia virus (ISAV); the co- linear transcript from Segment 7 probably encodes a non-structural or minor structural protein

Diseases of Aquatic Organisms

Bjørn Krossøy¹  Jegatheswaran Thevarajan¹  Maaike Joerink¹  Eirik Hoel¹  Are Nylund¹  Knut Falk¹  Curt Endresen¹  Eirik Biering¹ 

关键词: ISAV;    Splicing;    Matrix;    Segment 7;    Segment 8;    GenBank Accession No. AY044132;   

DOI  :  10.3354/dao049117

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: In this study we present the cloning, expression and partial identification of Genomic Segment 7 of infectious salmon anaemia virus (ISAV). The nucleotide sequence corresponding to Segment 7 was isolated from a bacteriophage lambda cDNA libraryand contained 2 overlapping open reading frames (ORFs) of 903 and 522 bases respectively. It also contained an ISAV-specific conserved nucleotide motif in the mRNA 5¹ region. The co-linear transcript representing the large ORF undergoes a splicing eventthat removes a 526 nucleotide intron to form a mRNA corresponding to the smaller reading frame. Thus, ISAV Genomic Segment 7 has a similar coding strategy as influenza A virus Segments 7 and 8. The largest ORF of Segment 7 and the first ORF of Segment 8was expressed in E. coli as fusion proteins and rabbit antiserum was raised against the recombinant protein from Segment 8. Immunoblot studies using this antiserum and a serum against purified virus, show that Segment 8 encodes one of the majorstructural proteins of the virus whereas the co-linear ORF of Segment 7 probably encodes a non- or minor structural protein

【 授权许可】

Unknown