The Porcine Mitochondrial Transcription Factor a Gene: Molecular Characterization, Radiation Hybrid Mapping and Genetic Diversity among 12 Pig Breeds | Science Publications

American Journal of Animal and Veterinary Sciences

Peter Dovc¹  Jennifer J. Michal¹  Tanja Kunej¹  Zhihua M. Jiang¹  Xiao-Lin Wu¹  Tamara Berlic¹ 

关键词: alternative splicing;    annotation;    diversity;    mapping;    pig;    TFAM;   

DOI  :  10.3844/ajavsp.2009.129.135

学科分类：兽医学

来源: Science Publications

【 摘 要 】

Problem statement: Mitochondrial transcription factor A (TFAM) is a nucleus-encoded protein that is a key activator of mitochondrial transcription as well as a major participant in mitochondrial genome replication. Genomic characterization of the porcine TFAM gene is, therefore, necessary to determine its involvement in regulation of fat depots and meat quality traits in pigs. Approach: Genomic DNA sequence was determined using a comparative in silico annotation approach. RT-PCR was used for analysis of alternative splicing. Genome location was determined using Radiation Hybrid (RH) mapping. Genetic marker was identified by sequencing and genotyped by the PCR-RFLP method with SacI. GENEPOP version 3.3 software was used for statistic analysis. Results: We determined both full-length cDNA and genomic DNA sequences of the porcine TFAM gene. Gene expression analysis revealed an alternative 5

【 授权许可】

Unknown