The pathogenic potential of Helicobacter cinaedi isolated from non-human sources: adherence, invasion and translocation ability in polarized intestinal epithelial Caco-2 cells in vitro

Journal of Veterinary Medical Science

Yuji SAEKI²  Naoaki MISAWA¹  Wataru YAMAZAKI¹  Ichiro TAKAJO³  Takako TANIGUCHI¹  Tetsuya HAYASHI⁴  Akihiko OKAYAMA³ 

[1] Laboratory of Veterinary Public Health, Department of Veterinary Medical Science, Faculty of Agriculture, University of Miyazaki, 1�?1 Gakuenkibanadai-nishi, Miyazaki 889�?2192, Japan;Clinical Laboratory, University of Miyazaki Hospital, 5200 Kiyotake, Miyazaki 889�?1692, Japan;Department of Rheumatology, Infectious Diseases and Laboratory Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889�?1692, Japan;Division of Microbiology, Department of Infectious Diseases, Faculty of Medicine, University of Miyazaki, 5200 Kiyotake, Miyazaki 889�?1692, Japan

关键词: adherence;    Caco-2 cell;    Helicobacter cinaedi;    invasion;    translocation;   

DOI  :  10.1292/jvms.15-0595

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(2)Helicobacter cinaedi infection has been recognized as an increasingly important emerging disease in humans. Infection with H. cinaedi causes bacteremia, cellulitis and enteritis. H. cinaedi has been isolated from non-human sources, including dogs, cats and rodents; however, it remains unclear whether animal strains are pathogenic in humans and as zoonotic pathogens. In this study, H. cinaedi isolates were recovered from a dog and a hamster, and the ability of these isolates to adhere to, invade and translocate across polarized human intestinal epithelial Caco-2 cells was examined in vitro. To better understand the pathogenic potential of animal H. cinaedi isolates, these results were compared with those for a human strain that was isolated from a patient with bacteremia. The animal and human strains adhered to and invaded Caco-2 cells, but to a lesser degree than the C. jejuni 81�?176 strain, which was used as a control. The integrity of tight junctions was monitored by measuring transepithelial electrical resistance (TER) with a membrane insert system. The TER values for all H. cinaedi strains did not change during the experimental periods compared with those of the controls; however, translocation of H. cinaedi from the apical side to the basolateral side was confirmed by cultivation and H. cinaedi-specific PCR, suggesting that the H. cinaedi strains translocated by transcellular route. This study demonstrated that H. cinaedi strains of animal origin might have a pathogenic potential in human epithelial cells as observed in a translocation assay in vitro with a human isolate.

【 授权许可】

Unknown