Evaluation of the Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) as a Screening Method for the Detection of Influenza Viruses in the Fecal Materials of Water Birds

Journal of Veterinary Medical Science

Hidetoshi KANDA²  Syouhei SENBA²  Naoki YAMAMOTO¹  Hiroshi KIDA²  Yoshihiro SAKODA¹  Masatoshi OKAMATSU¹  Takahiro SOEJIMA²  Fumi YOSHINO¹  Hiromi YOSHIDA¹  Masayuki MOTOSHIMA¹  Mayumi ENDO¹ 

[1] Laboratory of Microbiology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University;Eiken Chemical Company, Limited

关键词: fecal material;    influenza virus;    reverses transcription loop-mediated isothermal amplification (RT-LAMP);    screening;    surveillance;   

DOI  :  10.1292/jvms.10-0505

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(29)Cited-By(8)Migratory water birds are a natural reservoir for influenza A viruses. Viruses replicate in the intestines of ducks and are shed with the fecal materials. Virus isolation from collected fecal materials, therefore, is an integral part of the surveillance of avian influenza in water birds. In the present study, reverse transcription loop-mediated isothermal amplification (RT-LAMP) was assessed for its usefulness in detecting the RNA of influenza A viruses in fecal materials. It was found that, RT-LAMP specifically and sensitively detects the matrix gene of influenza A viruses. Influenza A viruses were isolated from the fecal materials in which viral RNA were detected by RT-LAMP in 35 min. The present findings indicate that RT-LAMP is useful as a high throughput screening method for field samples prior to virus isolation, allowing the processing of hundreds of samples per day.

【 授权许可】

Unknown