LAT1 Is a Central Transporter of Essential Amino Acids in Human Umbilical Vein Endothelial Cells

Journal of Pharmacological Sciences

Takao Kamai³  Promsuk Jutabha¹  Naohiko Anzai¹  Hitoshi Endou²  Keitaro Hayashi¹ 

[1] Department of Pharmacology and Toxicology, Dokkyo Medical University School of Medicine, Japan;J-Pharma Co., Ltd., Japan;Department of Urology, Dokkyo Medical University School of Medicine, Japan

关键词: LAT1;    amino acid transporter;    HUVEC;   

DOI  :  10.1254/jphs.13255SC

学科分类：药学

来源: Nihon Yakuri Gakkai Henshuubu / Japanese Pharmacological Society

【 摘 要 】

References(8)Cited-By(2)Endothelial cell proliferation supporting angiogenesis requires sufficient nutrient supply because of facilitated intracellular metabolism. However, little is known about the mechanism for the promotion of nutrient incorporation in proliferating endothelial cells. Here we show that L-type amino acid transporter 1 (LAT1) is a major transporter of essential amino acids in human umbilical vein endothelial cells (HUVECs). Growing HUVECs express a certain level of LAT1. A LAT1-specific inhibitor suppressed leucine uptake, cell proliferation, and tube formation of HUVECs. Therefore, LAT1 acts to support effective uptake of amino acids, which is critical for the optimal function of HUVECs for angiogenesis.

【 授权许可】

Unknown