Immunohistochemical Analysis of Expression Patterns of Tumor Necrosis Factor Receptors on Lymphoma Cells in Enzootic Bovine Leukosis

Journal of Veterinary Medical Science

Naotaka ISHIGURO¹  Kosuke OKADA¹  Satoru KONNAI²  Manabu IKEDA¹  Masanobu GORYO¹  Misao ONUMA² 

[1] Department of Pathogenic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University;Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University

关键词: enzootic bovine leukosis;    lymphoma;    phenotype;    tumor necrosis factor-α;    tumor necrosis factor receptor;   

DOI  :  10.1292/jvms.67.425

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(47)Cited-By(2)Tumor necrosis factor-alpha (TNF-α) has been reported to be associated with the progression of lymphoproliferative neoplastic diseases and retroviral infections. Hence we examined immunohistochemically the expression patterns of TNF-receptors (TNF-RI and RII) on lymphoma cells derived from the 29 cases of enzootic bovine leukosis (EBL). Lymphomas obtained in 29 animals with EBL were histopathologically classified into three types: diffuse mixed type (10 cases), diffuse large type (9 cases), and diffuse large cleaved type (10 cases). Immunohistochemically using a monoclonal antibody to a bovine lymphocyte surface antigen, the lymphomas were classified into three phenotypes: B-1a (CD5+/CD11b+), B-1b (CD5-/CD11b+) and B-2 (conventional B) (CD5-/CD11b-). Interestingly, the lymphoma cells in all animals expressed TNF-RII, but not TNF-RI. Although, in EBL, lymphoma cells of which the histopathological and immunological property differs has been formed, the expression patterns of TNF-Rs had the universality in all lymphoma cells. TNF-RII, which induces cell proliferation, was expressed but TNF-RI, which induces cell apoptosis was not expressed on all lymphoma cells, suggesting that TNF-Rs play an important role in the malignant proliferation of B cells and formation of lymphomas in EBL.

【 授权许可】

Unknown