Generation of Neuronal-Like Cells from Umbilical Cord Blood-Derived Mesenchymal Stem Cells of a RFP-Transgenic Cloned Cat

Journal of Veterinary Medical Science

Guang-Zhen JIN³  Xi-Jun YIN²  Il-Keun KONG³  Sung-Tae YEE¹  Young-S LEE³  Eu-Gene CHOI³  Jin-Tae JEON³  Su-Jin CHO³  Xian-Feng YU² 

[1] Department of Biology, Sunchon National University;Department of Animal Science & Technology, Sunchon National University;Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University

关键词: differentiation;    mesenchymal stem cell;    neuronal-like cell;   

DOI  :  10.1292/jvms.70.723

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(21)Cited-By(10)Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiation into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronal-induction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were β III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A β III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation.

【 授权许可】

Unknown