Immunophenotypic characterization of patients with acute myeloid leukemia

Revista Cubana de Hematología, Inmunología y Hemoterapia

Pino Blanco, Daily¹  Macías Abraham, Consuelo¹  Lahera Sánchez, Tania²  Socarrás Ferrer, Berta B¹  Marsán Suárez, Vianed¹  del Valle Pérez, Lázaro O¹  Martínez Machado, Mercedes¹  Sánchez Segura, Miriam de la C¹ 

[1] Instituto de Hematología e Inmunología, La Habana, Cuba;Instituto Nacional de Oncología y Radiobiología, La Habana, Cuba

关键词: immunophenotyping;    myeloid leukemia;    monoclonal antibodies;    malignant hemopathies;   

DOI  :  

学科分类：生理学与病理学

来源: SciELO

【 摘 要 】

Introduction: Acute myeloid leukemia (AML) is a heterogeneous group of diseases characterized by clonal expansion of myeloblasts; its classification involves several criteria, including the immunological one.Objective: To characterize the immunophenotype of patients with acute myeloid leukemia who were evaluated at the Institute of Hematology and Immunology.Methods: A descriptive study of patients diagnosed with this kind of leukemia, whose blood samples were processed at the Department of Immunology during 2008-2012. An immunocytochemical ultramicromethod that uses a panel of monoclonal antibodies specific for myeloid and lymphoid antigens was applied. The variables analyzed were age, sex, subtype of leukemia and expression of immunological markers; their association was analyzed with the Chi-square test and Spearman's rank correlation coefficient.Results: The study covered 58 patients; 28 were males and 30 females. The predominant age group was 0 - 9 years with a median of 26 years. M4 subtype was the most common (30,4%). M4 and M7 subtypes predominated in children, while M0 predominated in adults with statistically significant differences (p d» 0,05). The combination of pan-myeloid antigens CD13 and CD33 was present in 91% of patients. Combinations of CD13/CD33, CD14/CD15, CD33/CD14 and CD33/CD15 showed significant correlation. In 20,6 % of patients tested, lymphoid antigens were also detected. In this group, no significant differences by gender and age. CD7 antigen was the most expressed followed by antigens: CD3, CD20, CD22 and CD79, in equal proportion.Conclusions: Immunophenotyping of leukemia cells appeared as a useful tool to confirm the morphological and clinical diagnosis of acute myeloid leukemia.

【 授权许可】

Unknown