Secretion of Inhibin during the Estrous Cycle in the Female Asian Elephant (Elephas maximus)

Journal of Veterinary Medical Science

Saroch KAEWMANEE¹  Teruyuki KOMIYA²  Yuki YAMAMOTO¹  Miori KISHIMOTO³  Tatsuya YAMAMOTO¹  Wan Zhu JIN³  Etsuo NARUSHIMA⁴  Gen WATANABE¹  Kentaro NAGAOKA³  Kazuyoshi TAYA¹ 

[1] Department of Basic Veterinary Science, The United Graduate School of Veterinary Sciences, Gifu University;Ueno Zoological Gardens;Laboratory of Veterinary Physiology, Department of Veterinary Medicine, Faculty of Agriculture, Tokyo University of Agriculture and Technology;Tama Zoological Park

关键词: Asian elephant;    corpora lutea;    follicles;    inhibin;    progesterone;   

DOI  :  10.1292/jvms.10-0267

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(30)Cited-By(2)To define the source of circulating inhibin in female Asian elephants, the immunolocalizations of the inhibin α, βA and βB subunits, 3β-hydroxysteroid dehydrogenase (3β-HSD), aromatase cytochrome P450 (P450arom) and cytochrome 17α-hydroxylase P450 (P450 c17) were investigated. Concentrations of immunoreactive (ir-) inhibin, progesterone and follicle-stimulating hormone (FSH) during the estrous cycle were measured by radioimmunoassay. Inhibin immunoreactivity in follicular fluid and homogenate of corpora lutea was also measured. Immunolocalizations of inhibin subunits, 3β-HSD, P450arom and P450c17 were detected in the granulosa cells of antral follicles and luteal cells. The follicular fluid contained high levels of ir-inhibin and bioactive inhibin. The homogenate of corpora lutea also contained ir-inhibin. Serum ir-inhibin remained at low levels during the early non-luteal phase, began to increase from the late non-luteal phase and continued to increase during the early luteal phase. Serum ir-inhibin showed maximal levels in the middle of the luteal phase and gradually decreased to baseline three weeks prior to progesterone decline. The serum ir-inhibin levels were positively correlated with progesterone throughout the estrous cycle. On the other hand, ir-inhibin was negatively correlated with FSH during the late non-luteal and early luteal phases. These findings strongly suggest that the corpus luteum is one of the sources of inhibin as well as granulosa cells in the Asian elephant.

【 授权许可】

Unknown