Development of a monoclonal antibody specific to yellow head virus (YHV) from Penaeus monodon

Diseases of Aquatic Organisms

Paisarn Sithigorngul¹  Weerawan Sithigorngul¹  Parin Chaivisuthangkura¹  Piamsak Menasveta¹  -¹  Phudhi Chauychuwong¹  Siwaporn Longyant¹ 

关键词: Immunocytochemistry;    Monoclonal antibodies;    Penaeus monodon;    Yellow head virus (YHV);   

DOI  :  10.3354/dao042027

学科分类：生物科学（综合）

来源: Inter-Research

【 摘 要 】

ABSTRACT: A monoclonal antibody specific to yellow head virus (YHV) was produced from a mouse immunized with gill extracts prepared from laboratory-reared Penaeus monodon dually infected with YHV and white spot syndrome virus (WSSV). One clonedesignated V3-2B specifically bound to native and SDS-treated viral specific antigens. Immunocytochemical studies of infected gills revealed viral specific immunoreactivities in the cytoplasm of gill tissue and in haemocytes. No antibody binding wasobserved in gills from non-infected shrimp. In addition, immunocytochemical examination of tissues from shrimp experimentally infected with YHV gave a positive reaction, while tissues from uninfected control shrimp or shrimp experimentally infected withWSSV did not. Western blot analysis indicated that the antibody reacted with a protein of approximately 135 kD that was present only in shrimp infected with YHV. In dot-blot indirect immunoperoxidase assays, the antibody was able to detect viralassociated antigen in diluted haemolymph up to 1:50 dilution and in an ammonium sulfate precipitate of haemolymph up to 1:1000 dilution. The results suggested that this antibody might be useful for development of effective diagnostic techniques for bothheavy and mild YHV infections in shrimp.

【 授权许可】

Unknown