The Studies on the Transfection of EGFP-Fused Porcine Circovirus Type 2 Genome

Journal of Veterinary Medical Science

Shu-Hsiang YANG¹  Tsun-Yung KUO¹  Shiow-Suey LAI²  Hsu-Chung Gabriel CHEN² 

[1] Institute of Biotechnology, National Ilan University;School of Veterinary Medicine, National Taiwan University

关键词: EGFP-fused PCV2;    enhanced green fluorescent protein;    PCV2 DNA clone;   

DOI  :  10.1292/jvms.10-0490

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(11)Cited-By(1)This study described construction and transfection of an EGFP-fused Porcine Circovirus Type 2 (PCV2) genome and the recovery of the virus. Posttransfection, PCV2 (ORF1)-EGFP/pSK, PCV2 (ORF3)-EGFP/pSK, PCV2 (ORF4)-EGFP/pSK and PCV2(ORF5)-EGFP/pSK showed no fluorescent signals in transfected cells, while green fluorescent signals were observed in the nuclei of PK-15 cells after PCV2 (ORF2)-EGFP/pSK transfection. The presence of ORF2-EGFP fusion protein was demonstrated by dual signals of green fluorescence and anti-PCV2 antibodies conjugated with rhodamine in an immunofluorescence assay (IFA). Furthermore, the released EGFP-fused PCV2 genome was demonstrated by real-time PCR.

【 授权许可】

Unknown