Multiplexed imaging of immune cells in staged multiple sclerosis lesions by mass cytometry

eLife

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[1] Department of Immunology, University of Toronto, Toronto, Canada;Department of Neurology, Yale School of Medicine, New Haven, United States;Department of Neuroscience, Faculty of Medicine, Université de Montréal, Montreal, Canada;Fluidigm Canada Inc, Markham, Canada;STTARR Innovation Centre, University Health Network, Toronto, Canada;

关键词: imaging mass cytometry;    multiple sclerosis;    lesions;    immunopathology;    Human;   

DOI  :  10.7554/eLife.48051

来源: publisher

【 摘 要 】

10.7554/eLife.48051.001Multiple sclerosis (MS) is characterized by demyelinated and inflammatory lesions in the brain and spinal cord that are highly variable in terms of cellular content. Here, we used imaging mass cytometry (IMC) to enable the simultaneous imaging of 15+ proteins within staged MS lesions. To test the potential for IMC to discriminate between different types of lesions, we selected a case with severe rebound MS disease activity after natalizumab cessation. With post-acquisition analysis pipelines we were able to: (1) Discriminate demyelinating macrophages from the resident microglial pool; (2) Determine which types of lymphocytes reside closest to blood vessels; (3) Identify multiple subsets of T and B cells, and (4) Ascertain dynamics of T cell phenotypes vis-à-vis lesion type and location. We propose that IMC will enable a comprehensive analysis of single-cell phenotypes, their functional states and cell-cell interactions in relation to lesion morphometry and demyelinating activity in MS patients.

【 授权许可】

CC BY