Evolutionary emergence of Hairless as a novel component of the Notch signaling pathway

eLife

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[1] Bioinformatics and Genomics Unit, Center for Genomic Regulation, Barcelona, Spain;Division of Biological Sciences, Section of Cell and Developmental Biology, University of California, San Diego, La Jolla, United States;

关键词: evolutionary novelty;    suppressor of Hairless;    co-repressor recruitment;    metastasis-associated protein;    protein evolution;    developmental system drift;    D. melanogaster;   

DOI  :  10.7554/eLife.48115

来源: publisher

【 摘 要 】

10.7554/eLife.48115.001Suppressor of Hairless [Su(H)], the transcription factor at the end of the Notch pathway in Drosophila, utilizes the Hairless protein to recruit two co-repressors, Groucho (Gro) and C-terminal Binding Protein (CtBP), indirectly. Hairless is present only in the Pancrustacea, raising the question of how Su(H) in other protostomes gains repressive function. We show that Su(H) from a wide array of arthropods, molluscs, and annelids includes motifs that directly bind Gro and CtBP; thus, direct co-repressor recruitment is ancestral in the protostomes. How did Hairless come to replace this ancestral paradigm? Our discovery of a protein (S-CAP) in Myriapods and Chelicerates that contains a motif similar to the Su(H)-binding domain in Hairless has revealed a likely evolutionary connection between Hairless and Metastasis-associated (MTA) protein, a component of the NuRD complex. Sequence comparison and widely conserved microsynteny suggest that S-CAP and Hairless arose from a tandem duplication of an ancestral MTA gene.

【 授权许可】

CC BY