Stella modulates transcriptional and endogenous retrovirus programs during maternal-to-zygotic transition

eLife

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[1] European Molecular Biology Laboratory, European Bioinformatics Institute, Cambridge, United Kingdom;Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology, Daegu, Republic of Korea;European Molecular Biology Laboratory, European Bioinformatics Institute, Cambridge, United Kingdom;Wellcome Trust Sanger Institute, Cambridge, United Kingdom;Cancer Research United Kingdom Cambridge Institute, University of Cambridge, Cambridge, United Kingdom;Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom;Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom;Wellcome Trust/Cancer Research United Kingdom Gurdon Institute, University of Cambridge, Cambridge, United Kingdom;Department of Physiology, Development and Neuroscience, University of Cambridge, Cambridge, United Kingdom;European Molecular Biology Laboratory - Monterotondo, Rome, Italy;

关键词: maternal-to-zygotic transition;    Stella;    transposable elements;    chimeric transcripts;    MuERV-L/MERVL;    2-cell;    Mouse;   

DOI  :  10.7554/eLife.22345

来源: publisher

【 摘 要 】

10.7554/eLife.22345.001The maternal-to-zygotic transition (MZT) marks the period when the embryonic genome is activated and acquires control of development. Maternally inherited factors play a key role in this critical developmental process, which occurs at the 2-cell stage in mice. We investigated the function of the maternally inherited factor Stella (encoded by Dppa3) using single-cell/embryo approaches. We show that loss of maternal Stella results in widespread transcriptional mis-regulation and a partial failure of MZT. Strikingly, activation of endogenous retroviruses (ERVs) is significantly impaired in Stella maternal/zygotic knockout embryos, which in turn leads to a failure to upregulate chimeric transcripts. Amongst ERVs, MuERV-L activation is particularly affected by the absence of Stella, and direct in vivo knockdown of MuERV-L impacts the developmental potential of the embryo. We propose that Stella is involved in ensuring activation of ERVs, which themselves play a potentially key role during early development, either directly or through influencing embryonic gene expression.DOI: http://dx.doi.org/10.7554/eLife.22345.001

【 授权许可】

CC BY