Chronically implanted Neuropixels probes enable high-yield recordings in freely moving mice

eLife

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[1] Cold Spring Harbor Laboratory, Cold Spring Harbor, United States;Division of Biological Sciences, University of California, San Diego, San Diego, United States;University of Connecticut School of Medicine, Farmington, United States;

关键词: electrophysiology;    extracellular;    behavior;    Neuropixels;    Mouse;   

DOI  :  10.7554/eLife.47188

来源: publisher

【 摘 要 】

10.7554/eLife.47188.001The advent of high-yield electrophysiology using Neuropixels probes is now enabling researchers to simultaneously record hundreds of neurons with remarkably high signal to noise. However, these probes have not been well-suited to use in freely moving mice. It is critical to study neural activity in unrestricted animals for many reasons, such as leveraging ethological approaches to study neural circuits. We designed and implemented a novel device that allows Neuropixels probes to be customized for chronically implanted experiments in freely moving mice. We demonstrate the ease and utility of this approach in recording hundreds of neurons during an ethological behavior across weeks of experiments. We provide the technical drawings and procedures for other researchers to do the same. Importantly, our approach enables researchers to explant and reuse these valuable probes, a transformative step which has not been established for recordings with any type of chronically-implanted probe.

【 授权许可】

CC BY