Species specific differences in use of ANP32 proteins by influenza A virus

eLife

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[1] Influenza Viruses, The Pirbright Institute, Surrey, United Kingdom;Institute for Infection and Immunity, St. George's, University of London, London, United Kingdom;Section of Molecular Virology, Imperial College London, London, United Kingdom;The Roslin Institute, Royal (Dick) School of Veterinary Studies, University of Edinburgh, Midlothian, United Kingdom;

关键词: Influenza;    ANP32A;    ANP32B;    polymerase;    gene editing;    Chicken;    Human;    Virus;   

DOI  :  10.7554/eLife.45066

来源: publisher

【 摘 要 】

10.7554/eLife.45066.001Influenza A viruses (IAV) are subject to species barriers that prevent frequent zoonotic transmission and pandemics. One of these barriers is the poor activity of avian IAV polymerases in human cells. Differences between avian and mammalian ANP32 proteins underlie this host range barrier. Human ANP32A and ANP32B homologues both support function of human-adapted influenza polymerase but do not support efficient activity of avian IAV polymerase which requires avian ANP32A. We show here that the gene currently designated as avian ANP32B is evolutionarily distinct from mammalian ANP32B, and that chicken ANP32B does not support IAV polymerase activity even of human-adapted viruses. Consequently, IAV relies solely on chicken ANP32A to support its replication in chicken cells. Amino acids 129I and 130N, accounted for the inactivity of chicken ANP32B. Transfer of these residues to chicken ANP32A abolished support of IAV polymerase. Understanding ANP32 function will help develop antiviral strategies and aid the design of influenza virus resilient genome edited chickens.

【 授权许可】

CC BY