MagC, magnetic collection of ultrathin sections for volumetric correlative light and electron microscopy

eLife

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[1] Institute of Neuroinformatics, University of Zurich and ETH Zurich, Zurich, Switzerland;

关键词: ultramicrotomy;    correlative light and electron microscopy;    connectomics;    Zebra Finch;    Other;   

DOI  :  10.7554/eLife.45696

来源: publisher

【 摘 要 】

10.7554/eLife.45696.001The non-destructive collection of ultrathin sections on silicon wafers for post-embedding staining and volumetric correlative light and electron microscopy traditionally requires exquisite manual skills and is tedious and unreliable. In MagC introduced here, sample blocks are augmented with a magnetic resin enabling the remote actuation and collection of hundreds of sections on wafer. MagC allowed the correlative visualization of neuroanatomical tracers within their ultrastructural volumetric electron microscopy context.

【 授权许可】

CC BY