A combination of transcription factors mediates inducible interchromosomal contacts

eLife

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[1] Department of Genome Sciences, University of Washington, Seattle, United States;Department of Genome Sciences, University of Washington, Seattle, United States;Howard Hughes Medical Institute, Seattle, United States;Brotman Baty Institute for Precision Medicine, Seattle, United States;

关键词: saturation mutagenesis;    mutational scanning;    chromosome conformation;    3C;    homolog pairing;    transcription factors;    S. cerevisiae;   

DOI  :  10.7554/eLife.42499

来源: publisher

【 摘 要 】

10.7554/eLife.42499.001The genome forms specific three-dimensional contacts in response to cellular or environmental conditions. However, it remains largely unknown which proteins specify and mediate such contacts. Here we describe an assay, MAP-C (Mutation Analysis in Pools by Chromosome conformation capture), that simultaneously characterizes the effects of hundreds of cis or trans-acting mutations on a chromosomal contact. Using MAP-C, we show that inducible interchromosomal pairing between HAS1pr-TDA1pr alleles in saturated cultures of Saccharomyces yeast is mediated by three transcription factors, Leu3, Sdd4 (Ypr022c), and Rgt1. The coincident, combined binding of all three factors is strongest at the HAS1pr-TDA1pr locus and is also specific to saturated conditions. We applied MAP-C to further explore the biochemical mechanism of these contacts, and find they require the structured regulatory domain of Rgt1, but no known interaction partners of Rgt1. Altogether, our results demonstrate MAP-C as a powerful method for dissecting the mechanistic basis of chromosome conformation.

【 授权许可】

CC BY