Asymmetry between the two acidic patches dictates the direction of nucleosome sliding by the ISWI chromatin remodeler

eLife

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[1] TC Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, United States;

关键词: chromatin remodeler;    acidic patch;    nucleosome sliding;    Chd1;    ISWI;    SNF2h;    D. melanogaster;    Human;    S. cerevisiae;   

DOI  :  10.7554/eLife.45472

来源: publisher

【 摘 要 】

10.7554/eLife.45472.001The acidic patch is a functionally important epitope on each face of the nucleosome that affects chromatin remodeling. Although related by 2-fold symmetry of the nucleosome, each acidic patch is uniquely positioned relative to a bound remodeler. An open question is whether remodelers are distinctly responsive to each acidic patch. Previously we reported a method for homogeneously producing asymmetric nucleosomes with distinct H2A/H2B dimers (Levendosky et al., 2016). Here, we use this methodology to show that the Chd1 remodeler from Saccharomyces cerevisiae and ISWI remodelers from human and Drosophila have distinct spatial requirements for the acidic patch. Unlike Chd1, which is equally affected by entry- and exit-side mutations, ISWI remodelers strongly depend on the entry-side acidic patch. Remarkably, asymmetry in the two acidic patches stimulates ISWI to slide mononucleosomes off DNA ends, overriding the remodeler’s preference to shift the histone core toward longer flanking DNA.

【 授权许可】

CC BY