eLife | |
An arbitrary-spectrum spatial visual stimulator for vision research | |
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[1] Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany;Bernstein Center for Computational Neuroscience, University of Tübingen, Tübingen, Germany;Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany;Bernstein Center for Computational Neuroscience, University of Tübingen, Tübingen, Germany;Center for Integrative Neuroscience, University of Tübingen, Tübingen, Germany;Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany;Center for Integrative Neuroscience, University of Tübingen, Tübingen, Germany;Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany;Center for Integrative Neuroscience, University of Tübingen, Tübingen, Germany;Sussex Neuroscience, School of Life Sciences, University of Sussex, Falmer, United Kingdom;Institute for Ophthalmic Research, University of Tübingen, Tübingen, Germany;Sussex Neuroscience, School of Life Sciences, University of Sussex, Falmer, United Kingdom;Sussex Neuroscience, School of Life Sciences, University of Sussex, Falmer, United Kingdom; | |
关键词: color vision; visual system; retina; dichromatic vision; tetrachromatic vision; two-photon calcium imaging; Mouse; Zebrafish; | |
DOI : 10.7554/eLife.48779 | |
来源: publisher | |
【 摘 要 】
10.7554/eLife.48779.001Visual neuroscientists require accurate control of visual stimulation. However, few stimulator solutions simultaneously offer high spatio-temporal resolution and free control over the spectra of the light sources, because they rely on off-the-shelf technology developed for human trichromatic vision. Importantly, consumer displays fail to drive UV-shifted short wavelength-sensitive photoreceptors, which strongly contribute to visual behaviour in many animals, including mice, zebrafish and fruit flies. Moreover, many non-mammalian species feature more than three spectral photoreceptor types. Here, we present a flexible, spatial visual stimulator with up to six arbitrary spectrum chromatic channels. It combines a standard digital light processing engine with open source hard- and software that can be easily adapted to the experimentalist’s needs. We demonstrate the capability of this general visual stimulator experimentally in the in vitro mouse retinal whole-mount and the in vivo zebrafish. With this work, we intend to start a community effort of sharing and developing a common stimulator design for vision research.
【 授权许可】
CC BY
【 预 览 】
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RO201911193910670ZK.pdf | 4621KB | download |