Cross-species functional diversity within the PIN auxin efflux protein family

eLife

  ^{1    1    1    1    2    3} 

[1] The Sainsbury Laboratory, University of Cambridge, Cambridge, United Kingdom;United States Department of Energy Joint Genome Institute, Walnut Creek, United States;Department of Plant and Microbial Biology, University of California, Berkeley, Berkeley, United States;Western Regional Research Center, USDA-ARS, Albany, United States;

关键词: auxin;    Brachypodium;    evo-devo;    organ initiation;    polarity;    patterning;    A. thaliana;    Other;   

DOI  :  10.7554/eLife.31804

来源: publisher

【 摘 要 】

10.7554/eLife.31804.001In Arabidopsis, development during flowering is coordinated by transport of the hormone auxin mediated by polar-localized PIN-FORMED1 (AtPIN1). However Arabidopsis has lost a PIN clade sister to AtPIN1, Sister-of-PIN1 (SoPIN1), which is conserved in flowering plants. We previously proposed that the AtPIN1 organ initiation and vein patterning functions are split between the SoPIN1 and PIN1 clades in grasses. Here we show that in the grass Brachypodium sopin1 mutants have organ initiation defects similar to Arabidopsis atpin1, while loss of PIN1 function in Brachypodium has little effect on organ initiation but alters stem growth. Heterologous expression of Brachypodium SoPIN1 and PIN1b in Arabidopsis provides further evidence of functional specificity. SoPIN1 but not PIN1b can mediate flower formation in null atpin1 mutants, although both can complement a missense allele. The behavior of SoPIN1 and PIN1b in Arabidopsis illustrates how membrane and tissue-level accumulation, transport activity, and interaction contribute to PIN functional specificity.

【 授权许可】

CC BY