Cell-sized confinement controls generation and stability of a protein wave for spatiotemporal regulation in cells

eLife

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[1] Department of Basic Science, Komaba Institute for Science, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan;Department of Biosciences and Informatics, Keio University, Yokohama, Japan;Mathematical Science Group, WPI Advanced Institute for Materials Research (WPI-AIMR), Tohoku University Katahira, Sendai, Japan;MathAM-OIL, AIST, Sendai, Japan;

关键词: reaction-diffusion;    spatiotemporal regulation;    Min system;    artificial cells;    confinement;    protein wave;    E. coli;   

DOI  :  10.7554/eLife.44591

来源: publisher

【 摘 要 】

10.7554/eLife.44591.001The Min system, a system that determines the bacterial cell division plane, uses changes in the localization of proteins (a Min wave) that emerges by reaction-diffusion coupling. Although previous studies have shown that space sizes and boundaries modulate the shape and speed of Min waves, their effects on wave emergence were still elusive. Here, by using a microsized fully confined space to mimic live cells, we revealed that confinement changes the conditions for the emergence of Min waves. In the microsized space, an increased surface-to-volume ratio changed the localization efficiency of proteins on membranes, and therefore, suppression of the localization change was necessary for the stable generation of Min waves. Furthermore, we showed that the cell-sized space strictly limits parameters for wave emergence because confinement inhibits both the instability and excitability of the system. These results show that confinement of reaction-diffusion systems has the potential to control spatiotemporal patterns in live cells.

【 授权许可】

CC BY