The COMA complex interacts with Cse4 and positions Sli15/Ipl1 at the budding yeast inner kinetochore

eLife

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[1] Gene Center Munich, Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany;Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany;Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France;Laboratory of Chromosome Biology, Max Planck Institute of Biochemistry, Martinsried, Germany;Max F Perutz Laboratories, Medical University of Vienna, Vienna, Austria;Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Vienna, Austria;

关键词: kinetochore;    mass spectrometry;    feedback control;    chromosome segregation;    chemical crosslinking;    error correction;    chromosomal passenger complex;    aurora B;    S. cerevisiae;   

DOI  :  10.7554/eLife.42879

来源: publisher

【 摘 要 】

10.7554/eLife.42879.001Kinetochores are macromolecular protein complexes at centromeres that ensure accurate chromosome segregation by attaching chromosomes to spindle microtubules and integrating safeguard mechanisms. The inner kinetochore is assembled on CENP-A nucleosomes and has been implicated in establishing a kinetochore-associated pool of Aurora B kinase, a chromosomal passenger complex (CPC) subunit, which is essential for chromosome biorientation. By performing crosslink-guided in vitro reconstitution of budding yeast kinetochore complexes we showed that the Ame1/Okp1CENP-U/Q heterodimer, which forms the COMA complex with Ctf19/Mcm21CENP-P/O, selectively bound Cse4CENP-A nucleosomes through the Cse4 N-terminus. The Sli15/Ipl1INCENP/Aurora-B core-CPC interacted with COMA in vitro through the Ctf19 C-terminus whose deletion affected chromosome segregation fidelity in Sli15 wild-type cells. Tethering Sli15 to Ame1/Okp1 rescued synthetic lethality upon Ctf19 depletion in a Sli15 centromere-targeting deficient mutant. This study shows molecular characteristics of the point-centromere kinetochore architecture and suggests a role for the Ctf19 C-terminus in mediating CPC-binding and accurate chromosome segregation.

【 授权许可】

CC BY