Utilization of Extracted Protein from Fish Fin and Chicken Feather Waste for Alkaline Protease Production by Indigenous Bacteria

Jordan Journal of Biological Sciences

Raid D. Thanoon¹ 

关键词: Animal waste;    Environmental pollution;    Indigenous bacteria;    Protein Extraction;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Microbial Alkaline Proteases (APs) are of considerable interest in view of their activity and stability at alkaline pH. The present study aims to utilize keratin and collagen extracted from Fish Fin (FF) and Chicken Feather (CF) waste, respectively, for the production of AP enzyme by indigenous bacteria. Both wastes can be sources of solid waste contamination; hence, they were investigated for AP production through microbial degradation. The proteins extracted were added into the production medium containing the bacterial suspension, and assayed for AP production. The process parameters were optimized by One Factor At a Time (OFAT) and the optimum conditions for CF and FF were pH 9.0, temperature of 28°C for CF and 40°C for FF, incubation period was 6 and 10 days for CF and FF, respectively. The optimum carbon source was galactose and glucose for CF and FF, respectively, and the optimum nitrogen source was ammonium chloride and beef- extract, respectively. The inoculum size of 1.5 mL and a protein volume of 0.5 and 2.0 mL for CF and FF, respectively, was recorded. The present study indicates that the protein was successfully extracted from the waste used and degraded by AP enzyme that produced and optimized using OFAT by indigenous bacterial isolates.

【 授权许可】

CC BY