EVALUASI KERAGAMAN GENETIK SEMBILAN VARIETAS RAMBUTAN (Nephelium lappaceum) DENGAN MARKA RAPD - (Evaluation of Nine Rambutan (Nephelium lappaceum) Varieties Genetic Diversity Using RAPD Markers)

Biopropal Industri

Yuliana Galih Dyan Anggraheni^1¹  Enung Sri Mulyaningsih^2² 

[1] Pusat Penelitian Bioteknologi LIPI^1;Pusat Penelitian Bioteknologi-LIPI^2

关键词: genetic diversity;    molecular marker;    phylogenetic tree;    PIC value;    rambutan;   

DOI  :  

学科分类：化学工程（综合）

来源: Balai Riset dan Standardisasi Industri Pontianak

【 摘 要 】

High diversity of rambutan varieties is potensial to develop new improved rambutan varieties. Due to limitation of rambutan genetic diversity information, the usage of RAPD (Random Amplified Polymorphic DNA) markers becomes an efficient option. This research aimed to study the diversity of rambutan from the collection of LIPI germplasm garden genotypically. Nine rambutan varieties were analyzed using six RAPD markers which generated 45 bands. Polymorphism analysis showed variation between varieties 56,6% and PIC (Polymorphic Information Content) value 0,2-0,5. UPGMA (Unweighted Pair Group Method Arithmetic) dendogram divided nine rambutan varieties in two groups with similarity index of 85-95%. First group was Si Nyonya varieties and the second group consist of Rapiah, Aceh Lebak, Unidentified, Binjai, Parakan, Si Macan, Aceh Pagar and Lebak Bulus varieties. The group differences allegedly related to the stickiness of the flesh to the seed. Aceh Lebak varieties has the closest genetic relationship to unidentified varieties of 95%.

【 授权许可】

CC BY