Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar test

Plant, Soil and Environment

Alexander Ingo Linn¹ 

关键词: ALS inhibitor;    ACCase inhibitor;    herbicide-resistant weed;    rapid detection;    laboratory study;    PAM fluorimeter;   

DOI  :  10.17221/110/2018-PSE

学科分类：农业科学（综合）

来源: Czech Academy of Agricultural Sciences

【 摘 要 】

Reliable tests on herbicide resistance are important for resistance management. Despite well-established greenhouse bioassays, faster and in-season screening methods would aid in more efficient resistance detection. The feasibility of a chlorophyll fluorescence agar-based test on herbicide resistance in Apera spica-venti L. was investigated. Herbicide resistant and sensitive A. spica-venti seedlings were transplanted into agar containing pinoxaden and pyroxsulam herbicides. Chlorophyll fluorescence was measured and the maximum quantum efficiency of photosystem II (F _v /F _m ) was determined 48 h and 72 h after the transplantation to agar, respectively. The F _v /F _m values decreased with increasing herbicide concentration. Dose-response curves and respective ED ₅₀ values (herbicide concentration leading to 50% decrease of the F _v /F _m value) were calculated. However, each experiment repetition exhibited different sensitivities of the populations for both herbicides. In certain cases, resistant populations demonstrated similar F _v /F _m values as sensitive populations. Contrary to the findings in Alopecurus myosuroides Huds., discrimination of sensitive and resistant A. spica-venti populations was not feasible. An increased importance of the assessment time due to the herbicide concentrations calibrated for fast responses was assumed in this study.

【 授权许可】

CC BY