Differential Gene Expression of Durum Wheat (Triticum turgidum L. var. durum) in Relation to Genotypic Variation under NaCl Salinity Stress

Jordan Journal of Biological Sciences

Nidal Odat¹ 

关键词: Durum;    qRT-PCR;    Gene expression;    Genetic difference;    NaCl;    Wheat;    Jordan.;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Variation in expression of important genes under environmental stress is suggested to play an important role in determining phenotypic diversity. Virtually little is known about the genotypic-specific expression variation in durum wheat as an important cereal plant. This study examines transcript levels in durum (Triticum turgidum L.) using real-time PCR analysis in relation to genotypic differences by contrasting two gene pools of durum wheat under NaCl salinity stress. Four important genes in relation to stress were analyzed, three of which showed differential down-regulation of expression at the RNA level. Specifically, it is found that the expression of GAD and ASN genes showed a different pattern in response to different levels of salinity. At 50 mM NaCl GAD was down-regulated in only (Sham 1) genotype, while ASN was down-regulated in (Sham1), (Um Qais), and (Akcad). Under 100 mM NaCl, both genes were down-regulated in the genotype (Um Qais), while salinity of 150 mM NaCl induced down-regulation of both genes in (Um Qais) and (Norseh). Genetic variation (cultivars vs. landraces) was found to show a significant relationship to salinity responses especially at 50 and 150 mM NaCl (one-way ANOVA; P < 0.05). SOD and CAT genes were also down-regulated by salinity, but the relationship to genetic variation seems to be statistically less important. The genetic difference in gene expression could be best attributed to transcriptional and post-transcriptional regulation processes that are involved in durum tolerance to stress. This work demonstrates that the durum is an excellent plant system to study genetic variation in relation to gene expression under environmental stress because it seems that alleles of different genotypes of durum are affected by genetic background or environmental factors. The results of this study may help to classify the studied genotypes into sensitive and tolerant varieties.

【 授权许可】

CC BY