In Vitro Multiplication of the White Wormwood, Artemisia herba-alba asso

Jordan Journal of Biological Sciences

Rida A. Shibli¹ 

关键词: Artemisia herba-alba;    White wormwood;    Shih;    In vitro culture;    Micropropagation;    Seeds germination;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Artemisia herba-alba is a wild aromatic medicinal shrub which grows in the arid areas of North Africa and the Middle East. It is heavily subjected to loss and threats due to heavy grazing, cultivation and collection by the people who used it in folk medicine. In vitro propagation was experimented with different combinations of plant growth regulators to develop a suitable protocol to propagate this plant. In vitro microshoots of A. herba-alba were initiated from seeds. A maximum germination of seeds (94 %) was obtained using Paper-Bridge inserted in half Murashige and Skoog (MS) media and supplemented with 15 g/L of sucrose and 1.0 mg/L Gibberellic Acid (GA3). Proliferation of the in vitro plant was experimented at different concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg/L) of 6-Benzylaminopurine (BAP), kinetin, or 6-(gamma, gamma- Dimethylallylamino) purine (2iP). Rooting was experimented at different concentrations (0.0, 0.5, 1.0, 1.5 and 2.0 mg/L) of, Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) or alpha-Naphthalene acetic acid (NAA). The maximum proliferation of the A. herba-alba (23.6) microshoots was obtained when MS medium was supplemented with 1.0 mg/l 2iP and 1.5 mg/L GA3. The maximum root number (18.8) and length (14.08 cm) was achieved using 0.5 mg/L IBA. Both IAA and NAA failed to promote root induction. The rooted plants acclimatized successfully with 50 % of survival and were grown in the greenhouse. These results indicate the enormous potential of A. herba-alba for a large-scale multiplication, and it represents the first step to conserve its germplasm.

【 授权许可】

CC BY