The Corrective Efficacy of the n-Hexane Fraction of the Hydro-Methanol Extract of the Swietenia mahagoni Seeds on Testicular Dysfunctions in Streptozotocin-Induced Diabetic Male Rats

Jordan Journal of Biological Sciences

Tushar Kanti Bera¹ 

关键词: Swietenia mahagoni (L.) Jacq.;    Streptozotocin;    Antioxidant enzymes;    Pancreas;    Testis;   

DOI  :  

学科分类：生物科学（综合）

来源: Hashemite University, Deanship of Research and Higher Studies

【 摘 要 】

Diabetes mellitus (DM) is one of the most common chronic metabolic diseases worldwide affecting male reproductive dysfunctions (RD). Male diabetics are more prone to having varieties of sexual disorders such as impotence, retrograde ejaculation, sexual drive, decreased libido, delayed sexual maturation, and erectile dysfunction (ED). The present study is designed to investigate the diabetes-induced testicular dysfunction and its treatment by the n-hexane fraction of the hydro-methanolic (2:3) seed extract of Swietenia mahagoni in Wistar male albino rats. Diabetes was induced by the intramuscular injection of streptozotocin (STZ) at a dose of 4 mg/mL of citrate buffer/100 gm body weight. Diabetes-induced testicular oxidative stress injuries were assessed by the determination of antioxidant enzyme-activities including catalase (CAT), superoxide dismutase (SOD), and glutathione-s-transferase (GST), along with thiobarbituric acid-reactive substances (TBARS), and conjugated dienes (CD) levels. The numbers of the different generations of germ cells [i.e. spermatogonia-A (SgA), preleptotine spermatocytes (pLSc), mid-pachytene spermatocyte (mPSc), step 7 spermatids (7Sd) and 19 spermatids (19Sd)], at stage VII of spermatogenic cycle were assessed. Testicular CAT, SOD and GST activities were decreased along with elevation in the levels of TBARS and CD in diabetic animals. Numbers of different generations of germ cells i.e. SgA, pLSc, mPSc, 7Sd and 19Sd, at stage VII of the spermatogenic cycle were decreased in diabetic animals. Significant improvement in the levels of blood glucose, serum insulin, testosterone, and testicular oxidative stress parameters were noted towards control after the treatment of diabetic rats with the fraction at a dose of 10 mg/100 gm body weight/day for twenty-eight days. The above-mentioned different generations of germ cell numbers along with the diameter and number of pancreatic islets were recovered towards control after the treatment with the fraction. The results support the validity of this n-hexane fraction for the management of testicular disorders noted in diabetic rats.

【 授权许可】

CC BY