A high-throughput method for screening of L-tyrosine high-yield strains by Saccharomyces cerevisiae

The Journal of General and Applied Microbiology

Quanli Liu¹  Jiwei Mao²  Yuanzi Li³ 

[1] Department of Microbiology, College of Life Sciences, Nankai University;The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University;The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University,Department of Microbiology, College of Life Sciences, Nankai University

关键词: betaxanthin;    high-throughput screening;    L-tyrosine;    p-coumaric acid;    Saccharomyces cerevisiae;   

DOI  :  10.2323/jgam.2017.12.001

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

A biosensor screening assay based on the synthesis of betaxanthin was applied to relatively high throughput screening of the L-tyrosine mutant library. In the assays, fluorescence output showed a linear relationship between extracellular L-tyrosine content and yellow pigment formation. In addition, the yellow pigment accumulation of the L-tyrosine high-yield strain can be easily distinguished with the naked eye compared with the wild-type strain. As a result, numerous mutants that exhibited significantly increased coloration, were screened out after random mutagenesis, and p-coumaric acid production in mutants NK-A3 and NK-B4, were remarkably improved by 4-fold more than that of the wild-type strain. In general, this study provides a novel strategy for screening mutant libraries in the search for highly L-tyrosine-producing strains.

【 授权许可】

Unknown