Structural Variants and Selective Sweep Foci Contribute to Insecticide Resistance in the Drosophila Genetic Reference Panel

G3: Genes, Genomes, Genetics

Llewellyn Green^1¹  Pontus B. Leblanc^1²  Paul Battlay^1³ 

[1] Department of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig, 04103, Saxony, Germany^3;Gladstone Institute, University of California, San Francisco, CA, 94158^2;School of BioSciences, The University of Melbourne, Parkville, 3010, Victoria, Australia^1

关键词: Drosophila Genetic Reference Panel (DGRP);    malathion;    permethrin;    acetylcholinesterase;    Cyp6a17;   

DOI  :  10.1534/g3.118.200619

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Patterns of nucleotide polymorphism within populations of Drosophila melanogaster suggest that insecticides have been the selective agents driving the strongest recent bouts of positive selection. However, there is a need to explicitly link selective sweeps to the particular insecticide phenotypes that could plausibly account for the drastic selective responses that are observed in these non-target insects. Here, we screen the Drosophila Genetic Reference Panel with two common insecticides; malathion (an organophosphate) and permethrin (a pyrethroid). Genome-wide association studies map survival on malathion to two of the largest sweeps in the D. melanogaster genome; Ace and Cyp6g1 . Malathion survivorship also correlates with lines which have high levels of Cyp12d1 , Jheh1 and Jheh2 transcript abundance. Permethrin phenotypes map to the largest cluster of P450 genes in the Drosophila genome, however in contrast to a selective sweep driven by insecticide use, the derived allele seems to be associated with susceptibility. These results underscore previous findings that highlight the importance of structural variation to insecticide phenotypes: Cyp6g1 exhibits copy number variation and transposable element insertions, Cyp12d1 is tandemly duplicated, the Jheh loci are associated with a Bari1 transposable element insertion, and a Cyp6a17 deletion is associated with susceptibility.

【 授权许可】

CC BY