Comparative Oligo-FISH Mapping: An Efficient and Powerful Methodology To Reveal Karyotypic and Chromosomal Evolution

Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation

Jiming Jiang^8²  Hainan Zhao^3⁵  Jean-Marie Rouillard^6⁵  Guilherme T. Braz^1⁵  Kassandra Semrau^5⁶  Li He^2⁸  Tao Zhang^4^1,10  Giovana A. Torres^7^1,11 

[1] Arbor Biosciences, Ann Arbor, Michigan 48103;Arbor Biosciences, Ann Arbor, Michigan 48103^5;Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109^6;Department of Horticulture, Michigan State University, East Lansing, Michigan 48824^8;Department of Horticulture, University of Wisconsin–Madison, Wisconsin 53706;Department of Horticulture, University of Wisconsin–Madison, Wisconsin 53706^3;Department of Plant Biology, Michigan State University, East Lansing, Michigan 48824;Departmento de Biologia, Universidade Federal de Lavras, 37200, Brazil^1;Departmento de Biologia, Universidade Federal de Lavras, 37200, Brazil^7;Horticulture Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, China^2;Key Laboratory of Crop Genetics and Physiology of Jiangsu Province/Key Laboratory of Plant Functional Genomics of Ministry of Education, Yangzhou University, 225009, China^4

关键词: chromosome identification;    karyotype;    oligo-FISH;    chromosome painting;    translocation;   

DOI  :  10.1534/genetics.117.300344

学科分类：医学（综合）

来源: Genetics Society of America

【 摘 要 】

Developing the karyotype of a eukaryotic species relies on identification of individual chromosomes, which has been a major challenge for most nonmodel plant and animal species. We developed a novel chromosome identification system by selecting and labeling oligonucleotides (oligos) located in specific regions on every chromosome. We selected a set of 54,672 oligos (45 nt) based on single copy DNA sequences in the potato genome. These oligos generated 26 distinct FISH signals that can be used as a “bar code” or “banding pattern” to uniquely label each of the 12 chromosomes from both diploid and polyploid (4× and 6×) potato species. Remarkably, the same bar code can be used to identify the 12 homeologous chromosomes among distantly related Solanum species, including tomato and eggplant. Accurate karyotypes based on individually identified chromosomes were established in six Solanum species that have diverged for >15 MY. These six species have maintained a similar karyotype; however, modifications to the FISH signal bar code led to the discovery of two reciprocal chromosomal translocations in Solanum etuberosum and S. caripense . We also validated these translocations by oligo-based chromosome painting. We demonstrate that the oligo-based FISH techniques are powerful new tools for chromosome identification and karyotyping research, especially for nonmodel plant species.

【 授权许可】

CC BY