Split-QF System for Fine-Tuned Transgene Expression in Drosophila

Genetics: A Periodical Record of Investigations Bearing on Heredity and Variation

Olena Riabinina^1¹  Samuel W. Vernon^1² 

[1]Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Science Centre, University of Manchester, M13 9PL, United Kingdom^1

[2]Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, Virginia 21407^2

关键词: Q-system;    quinic acid;    split-GAL4;    split-LexA;   

DOI  :  10.1534/genetics.119.302034

学科分类：医学（综合）

来源: Genetics Society of America

【 摘 要 】

The Q-system is a binary expression system that works well across species. Here, we report the development and demonstrate the applications of a split-QF system that drives strong expression in Drosophila , is repressible by QS, and is inducible by a small nontoxic molecule (quinic acid). The split-QF system is fully compatible with existing split-GAL4 and split-LexA lines, thus greatly expanding the range of possible advanced intersectional experiments and anatomical, physiological, and behavioral assays in Drosophila , and in other organisms.

【 授权许可】

CC BY