Sex Determination in Ceratopteris richardii Is Accompanied by Transcriptome Changes That Drive Epigenetic Reprogramming of the Young Gametophyte

G3: Genes, Genomes, Genetics

Olga Vitek^2¹  Jo Ann Banks^5²  Federico Gaiti^3³  Nadia M. Atallah^1⁴  Milos Tanurdzic^4⁵ 

[1] College of Computer and Information Science, Northeastern University, Boston, MA 02115^2;Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47907^5;Department of Medicine, Weill Cornell Medicine and New York Genome Center, New York, NY 10013^3;Purdue University Center for Cancer Research^1;School of Biological Sciences, The University of Queensland, St. Lucia, Australia^4

关键词: sex determination;    RNA-seq;    Ceratopteris;    gametophyte;    epigenetics;    gibberellin;    antheridiogen;    transcriptome;    Genetics of Sex;   

DOI  :  10.1534/g3.118.200292

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

The fern Ceratopteris richardii is an important model for studies of sex determination and gamete differentiation in homosporous plants. Here we use RNA-seq to de novo assemble a transcriptome and identify genes differentially expressed in young gametophytes as their sex is determined by the presence or absence of the male-inducing pheromone called antheridiogen. Of the 1,163 consensus differentially expressed genes identified, the vast majority (1,030) are up-regulated in gametophytes treated with antheridiogen. GO term enrichment analyses of these DEGs reveals that a large number of genes involved in epigenetic reprogramming of the gametophyte genome are up-regulated by the pheromone. Additional hormone response and development genes are also up-regulated by the pheromone. This C. richardii gametophyte transcriptome and gene expression dataset will prove useful for studies focusing on sex determination and differentiation in plants.

【 授权许可】

CC BY