Transcriptional expression of PmrB and arn A within polymyxin-resistant nosocomial isolates of Pseudomonas aeruginosa from India

Journal of Microbiology and Infectious Diseases

Rajkumari Elizabeth^1¹  Sayani Roy^1^2^3²  Deepjyoti Paul^1^2^3³ 

[1] #Both Sayani Roy and Deepjyoti Paul have contributed equally and may be considered as First Author^3;Department of Microbiology, Assam University, Silchar, India^1;Department of Microbiology, Silchar Medical College and Hospital, Silchar, India^2

关键词: arn A;    mcr-1;    PmrB;    Polymyxin;    Pseudomonas aeruginosa;    Multidrug resistance;   

DOI  :  10.5799/jmid.434595

学科分类：生物科学（综合）

来源: Fatih University, Faculty of Medicine

【 摘 要 】

Objective: The polymyxin group of antibiotics is considered to be one of the most effective antimicrobial agents against many serious pathogenic bacteria, but the excessive use of these antibiotics has led to the development of drug resistance among bacteria. This study was designed to characterize polymyxin-resistant P. aeruginosa and to explore the role of PmrB and arn A in resistant phenotype. Methods: mRNA and cDNA of five selected polymyxin-resistant strains representing different MIC range; isolated under normal condition of strain growth, after treating sample/media with FeCl 3 and MgCl 2 alone, or after treating with FeCl 3 and polymyxin antibiotic. The transcriptional expression was observed for PmrB and arn A by quantitative real time RT-PCR in reference to P. aeruginosa PAO1. The presence of plasmid mediated colistin resistance determinants mcr-1 was screened by PCR. Susceptibility of the strains was determined by disc-diffusion method and DNA fingerprinting was carried out by performing REP-PCR. Results: A down regulated expression of PmrB and arn A was observed even after the unique induction with FeCl3 and MgCl2. All the isolates were found to be resistant against cefepime and different clonal patterns of resistance were found among the isolates. Conclusion: This study has drawn a new insight into polymyxin resistance which will help in the detection and control of infections caused by multidrug resistant P. aeruginosa . The low susceptibility rate to aminoglycoside, piperacllin-tazobactam and ciprofloxacin was found and in addition, detection of PmrB and arn A as molecular markers in the follow up of infections caused by multidrug resistant P. aeruginosa .

【 授权许可】

CC BY-NC