Anti-inflammation effects of 8-oxo-9-octadecenoic acid isolated from Undaria peterseniana in lipopolysaccharide-stimulated macrophage cells

EXCLI Journal

Seon-A Yoon¹  Su-Hyeon Cho²  Seung-Hae Kwon³  Min-Cheol Kang⁴  Young-Min Ham⁵  Soo-Jin Heo⁶ 

[1] Education, Korea Institute of Ocean Science &Technology (KIOST), Jeju 63349, Republic of Korea;Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 200-701, Republic of Korea;Department of Marine Life Sciences, Jeju National University, Jeju 690-756, Republic of Korea;Jeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP), Jeju 699-943, Republic of Korea;Jeju International Marine Science Center for Research &

关键词: 8-Oxo-9-octadecenoic acid;    anti-inflammatory;    Undaria peterseniana;    macrophages;    lipopolysaccharide;   

DOI  :  10.17179/excli2018-1422

学科分类：过敏症与临床免疫学

来源: University of Mainz, University of Leipzig

【 摘 要 】

The aim of this study was to investigate the anti-inflammatory activity of 8-oxo-9-octadecenoic acid (OOA) isolated from Undaria peterseniana by examining its ability to inhibit the lipopolysaccharide (LPS)-induced production of inflammatory mediators in RAW 264.7 macrophage cells. We found that OOA significantly suppressed the LPS-induced production of nitric oxide (NO) and inflammatory cytokines. OOA downregulated the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 proteins. With respect to proinflammatory signaling pathways, OOA inhibited LPS-induced mitogen-activated protein kinase signaling by inhibiting the phosphorylation of c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase (ERK). Moreover, OOA inhibited LPS-induced nuclear factor (NF)-κB signaling by reducing the phosphorylation of IκB-α and p50 proteins. These results indicate that OOA significantly reduces proinflammatory signaling, which results in reduced expression of cytokines and proinflammatory mediators. Taken together, these results suggest that OOA has potent anti-inflammatory effects and could be considered an effective anti-inflammatory agent.

【 授权许可】

CC BY