Identification of Chlamydia pneumoniae candidate genes that interact with human apoptotic factor caspase-9

The Journal of General and Applied Microbiology

Md. Abdul Aziz¹ 

[1] Graduate School of Biology-Oriented Science and Technology, Kindai University

关键词: apoptosis;    caspase-9;    Chlamydia pneumoniae;    Glycogen synthesis;    host-parasite interaction;    yeast two-hybrid;   

DOI  :  10.2323/jgam.2017.12.008

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

Chlamydia pneumoniae is an obligate intracellular pathogen responsible for respiratory diseases, including pneumonia and bronchitis, and is highly involved in chronic diseases, including atherosclerosis, asthma, and Alzheimer's disease. We previously showed that the host apoptotic factor caspase-9 played a crucial role for chlamydial multiplication and host apoptosis inhibition by chlamydial infection. To identify chlamydial genes interacting with human caspase-9, yeast two-hybrid screening was performed and 5 chlamydial genes, including Cpj0838 and pmpG were isolated from the C. pneumoniae genomic library. Pull-down experiments showed that caspase-9 physically bound to the Cpj0838 product and chlamydial cells, which contain PmpG proteins. This study could provide a clue to understanding host-Chlamydia interactions, especially the apoptosis repression by Chlamydia infection.

【 授权许可】

Unknown