TP901-1 Phage Recombinase Facilitates Genome Engineering in Drosophila melanogaster

G3: Genes, Genomes, Genetics

Roumen Voutev^1¹ 

[1] Department of Biochemistry and Molecular Biophysics and Systems Biology, Columbia University, Zuckerman Mind Brain Behavior Institute, 3227 Broadway, Quad 9A, New York, NY 10027^1

关键词: TP901-1;    site-directed recombinase;    cassette exchange;    CRISPR/Cas9;    genome engineering;    Drosophila melanogaster;   

DOI  :  10.1534/g3.119.0002

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Molecular biology techniques have a large impact on biomedical research and the availability of diverse tools to perform genome manipulations advances the ease of executing complicated genetic research. Here, we introduce in the fruit fly another such tool by harnessing the phage recombinase TP901-1 to perform site-directed recombination that leads to recombinase-mediated cassette exchange (RMCE). The TP901-1 system complements already existing recombination systems and enhances genome engineering in the fruit fly and other organisms.

【 授权许可】

CC BY