A Novel Niosomal Combination of Selenium Coupled with Glucantime against Leishmania tropica

The Korean Journal of Parasitology

Iraj Sharifi^1¹  Payam Khazaeli^2²  Saeedeh Farajzadeh^3³  Mahshid Mostafavi^1⁴ 

[1] Department of Pediatric Dermatology, Kerman University of Medical Sciences, Kerman, Iran^3;Department of Pharmaceutics, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran^2;HIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran^4;Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran^1

关键词: Leishmania tropica;    selenium;    glucantime;    niosomes;    anti-leishmanial effect;    cytokine;   

DOI  :  10.3347/kjp.2019.57.1.1

学科分类：生物科学（综合）

来源: The Korean Society for Parasitology

【 摘 要 】

There is no effective treatment modality available against different forms of leishmaniasis. Therefore, the aim of this study was to improve the penetration and efficacy of selenium and glucantime coupled with niosomes and compared them with their simple forms alone on in vitro susceptibility assays. In this study, the niosomal formulations of selenium and in combination with glucantime were prepared. The size and morphology of the niosomal formulations were characterized and the effectivity of the new formulation was also evaluated using in vitro MTT assay, intra-macrophage model, and gene expression profile. From the results obtained, no cytotoxicity effect was observed for niosomal and simple forms of drugs, as alone or in combination. Niosomal formulations of the drugs significantly showed more inhibitory effects ( P ≤0.001) than the simple drugs when the selectivity index was considered. The gene expression levels of Interleukin (IL-10) significantly decreased, while the level of IL-12 and metacaspase significantly increased ( P ≤0.001). The results of the present study showed that selenium plus glucantime niosome possess a potent anti-leishmanial effect and enhanced their lethal activity as evidenced by the in vitro experiments.

【 授权许可】

Free