In-vitro cytotoxic activities of poly(2-ethyl-2-oxazoline)-based amphiphilic block copolymers prepared by CuAAC click chemistry

eXPRESS Polymer Letters

S. Gulyuz¹ 

关键词: polymer synthesis;    molecular engineering;    amphiphilic block copolymers;    copper-catalyzed azide-alkyne cycloaddition;    click chemistry;    in-vitro cytotoxic activity;    polyoxazolines;   

DOI  :  10.3144/expresspolymlett.2018.13

学科分类：生物科学（综合）

来源: eXPRESS Polymer Letters

【 摘 要 】

Synthesis and characterization of well-defined amphiphilic block copolymers containing poly(2-ethyl-2-oxazoline)as hydrophilic block and poly(ε-caprolactone) or poly(L-lactide) as hydrophobic block is achieved by copper-catalyzedazide-alkyne cycloaddition (CuAAC) click chemistry. The clickable precursors, α-alkyne-functionalized poly(ε-caprolactone)and poly(L-lactide) and ω-azido-functionalized poly(2-ethyl-2-oxazoline) are simply prepared and joined using copper sulfate/ascorbic acid catalyst system at room temperature. The structures of precursors and amphiphilic block copolymers arecharacterized by spectroscopic, chromatographic and thermal analyses. The cytotoxic activities of resulting amphiphilicblock copolymers and their precursors are investigated in the prostate epithelial and cancer cells under in-vitro conditions.The treatment of the healthy prostate epithelial cell line PNT1A reveals that no significant cytotoxicity, whereas some significanttoxic effects on the prostate cancer cell lines are observed.

【 授权许可】

CC BY