Novel exc Genes Involved in Formation of the Tubular Excretory Canals of Caenorhabditis elegans

G3: Genes, Genomes, Genetics

Hikmat Al-Hashimi^1¹ 

[1] Dept. of Molecular Biosciences, University of Kansas, Lawrence, KS, 66045, USA^1

关键词: Tubulogenesis;    lumen formation;    endosomes;    EXC-5;    Charcot-Marie-Tooth Syndrome Type 4H;   

DOI  :  10.1534/g3.119.200626

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Regulation of luminal diameter is critical to the function of small single-celled tubes, of which the seamless tubular excretory canals of Caenorhabditis elegans provide a tractable genetic model. Mutations in several sets of genes exhibit the Exc phenotype, in which canal luminal growth is visibly altered. Here, a focused reverse genomic screen of genes highly expressed in the canals found 18 genes that significantly affect luminal outgrowth or diameter. These genes encode novel proteins as well as highly conserved proteins involved in processes including gene expression, cytoskeletal regulation, and vesicular and transmembrane transport. In addition, two genes act as suppressors on a pathway of conserved genes whose products mediate vesicle movement from early to recycling endosomes. The results provide new tools for understanding the integration of cytoplasmic structure and physiology in forming and maintaining the narrow diameter of single-cell tubules.

【 授权许可】

CC BY