Development of Diagnostic SNP Markers To Monitor Hybridization Between Sika Deer (Cervus nippon) and Wapiti (Cervus elaphus)

G3: Genes, Genomes, Genetics

Hengxing Ba^1¹ 

[1] State Key Laboratory for Molecular Biology of Special Wild Economic Animals, Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Jingyue District, Changchun, Jilin province, 130112, P.R. China^1

关键词: Key words: sika deer;    wapiti;    hybridization;    ddRAD-seq;    SNP discovery;    STACKS software;   

DOI  :  10.1534/g3.118.200417

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

Sika deer ( Cervus Nippon ) and wapiti ( Cervus elaphus ) are closely related species and their hybridization can result in significant allele-shift of their gene pool. Additive genetic effects and putative heterotic effects of their hybridization on growth performance could confer considerable economic advantage in deer farming. Here, we used double-digest restriction site-associated DNA sequencing technology (ddRAD-seq) and detected ∼320,000 genome-wide SNPs from 30 captive individuals: 7 sika deer, 6 wapiti and 17 F1 hybrids (reciprocal cross). By screening observed heterozygosity of each SNP across four taxonomic groups, we report for the first time a resource of 2,015 putative diagnostic SNP markers (species-specific SNPs for sika deer and wapiti), which can be used to design tools for assessing or monitoring the degree of hybridization between sika deer and wapiti. These ddRAD-seq data and SNP datasets are also valuable resources for genome-wide studies, including trait discovery for breeders of domestic deer.

【 授权许可】

CC BY