Characterization of mycosporine-like amino acids in the cyanobacterium Nostoc verrucosum

The Journal of General and Applied Microbiology

Kaori Inoue-Sakamoto¹  Yu Kanesaki²  Chieri Tsuji³  Tomoya Asano⁴  Ehsan Nazifi⁵  Hirofumi Yoshikawa⁶  Seiichi Matsugo⁷  Kenji Ishihara⁸  Takumi Nishiuchi⁹ 

[1]Department of Applied Bioscience, College of Bioscience and Chemistry, Kanazawa Institute of Technology

[2]Department of Bioscience, Tokyo University of Agriculture

[3]Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University

[4]Division of Functional Genomics, Advanced Science Research Center, Kanazawa University

[5]Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University

[6]Division of Life Science, Graduate School of Natural Science and Technology, Kanazawa University,Division of Biological Sciences, Graduate School of Natural Science and Technology, Kanazawa University,School of Natural System, College of Science and Engineering, Kanazawa University

[7]Marine Biochemistry Division, National Research Institute of Fisheries Science

[8]NODAI Genome Research Center, Tokyo University of Agriculture

[9]School of Natural System, College of Science and Engineering, Kanazawa University

关键词: antioxidant;    environmental adaptation;    mycosporine-like amino acid (MAA);    mys genes;   

DOI  :  10.2323/jgam.2017.12.003

学科分类：微生物学和免疫学

来源: Applied Microbiology, Molecular and Cellulrar Biosciences Research Foundation

【 摘 要 】

The aquatic cyanobacterium Nostoc verrucosum forms macroscopic colonies in streams, and its appearance is superficially similar to that of the terrestrial cyanobacterium Nostoc commune. N. verrucosum is sensitive to desiccation, unlike N. commune, although these Nostoc cyanobacterial species share physiological features, including massive extracellular polysaccharide production and trehalose accumulation capability. In this study, water-soluble sunscreen pigments of mycosporine-like amino acids (MAAs) were characterized in N. verrucosum, and the mysABCD genes responsible for MAA biosynthesis in N. verrucosum and N. commune were compared. N. verrucosum produced porphyra-334 and shinorine, with porphyra-334 accounting for >90% of the total MAAs. Interestingly, porphyra-334 is an atypical cyanobacteial MAA, whereas shinorine is known as a common and dominant MAA in cyanobacteria. Porphyra-334 from N. verrucosum showed little or no radical scavenging activity in vitro, although the glycosylated derivatives of porphyra-334 from N. commune are potent radical scavengers. The presence of the mysABCD gene cluster in N. commune strain KU002 (genotype A) supported its porphyra-334 producing capability via the Nostoc-type mechanism, although the genotype A of N. commune mainly produces the arabinose-bound porphyra-334. The mysABC gene cluster was conserved in N. verrucosum, but the mysD gene was not included in the cluster. These results suggest that the mysABCD gene products are involved in the biosynthesis of porphyra-334 commonly in these Nostoc species, and that the genotype A of N. commune additionally acquired the glycosylation of porphyra-334.

【 授权许可】

Unknown