Composite beads of silica gel, alginate and poly(aspartic acid) for the immobilization of a lipase enzyme

eXPRESS Polymer Letters

E. Krisch¹ 

关键词: polymer composites;    biodegradable polymers;    biocatalysis;    enzyme;    kinetic resolution;   

DOI  :  10.3144/expresspolymlett.2019.43

学科分类：生物科学（综合）

来源: eXPRESS Polymer Letters

【 摘 要 】

Silica gel/alginate/poly(aspartic acid) composite beads were prepared for immobilization of lipase B enzyme fromCandida antarctica (CaLB). CaLB was first adsorbed on functionalized mesoporous silica gel particles, which were thenentrapped in the interpenetrating network of thiolated poly(aspartic acid) and alginate, cross-linked by zinc ions. Finally,the beads were chemically stabilized by poly(ethylene glycol) diglycidyl ether, a bisepoxide cross-linker. In this manner,spherical biocatalysts with a diameter of 3–4 mm were prepared and their biocatalytic performance was tested by kineticresolution of racemic 1-phenylethanol. The activity of CaLB in the beads was comparable to that of CaLB physically adsorbedon silica gel particles. The composite beads were easy to recover after use and no loss of biocatalytic activity wasobserved even after five test reaction cycles. Furthermore, the CaLB in the composite beads showed sufficient thermal stabilityup to 90 °C, contrary to CaLB adsorbed only on silica gel particles.

【 授权许可】

CC BY