RNA-Seq-Based Analysis Reveals Heterogeneity in Mature 16S rRNA 3′ Termini and Extended Anti-Shine-Dalgarno Motifs in Bacterial Species

G3: Genes, Genomes, Genetics

Jordan R. Silke^1¹ 

[1] Ottawa Institute of Systems Biology, Ottawa, Ontario, Canada K1H 8M5^1

关键词: Gene Expression;    RNA-Seq;    Translation Initiation;   

DOI  :  10.1534/g3.118.200729

学科分类：生物科学（综合）

来源: Genetics Society of America

【 摘 要 】

We present an RNA-Seq based approach to map 3′ end sequences of mature 16S rRNA (3′ TAIL) in bacteria with single-base specificity. Our results show that 3′ TAILs are heterogeneous among species; they contain the core CCUCC anti-Shine-Dalgarno motif, but vary in downstream lengths. Importantly, our findings rectify the mis-annotated 16S rRNAs in 11 out of 13 bacterial species studied herein (covering Cyanobacteria, Deinococcus-Thermus, Firmicutes, Proteobacteria, Tenericutes, and Spirochaetes). Furthermore, our results show that species-specific 3′ TAIL boundaries are retained due to their high complementarity with preferred Shine-Dalgarno sequences, suggesting that 3′ TAIL bases downstream of the canonical CCUCC motif play a more important role in translation initiation than previously reported.

【 授权许可】

CC BY