First evidence of Zika virus venereal transmission in Aedes aegypti mosquitoes

Memorias do Instituto Oswaldo Cruz

Belchior, Heliana Christy Matos^2³  Almeida, Jéssica Feijó^1^1,11  Naveca, Felipe Gomes^2^5,50  Nascimento, Valdinete Alves do^2^6,68  Ríos-Velásquez, Claudia María^2^7,72  Pessoa, Felipe Arley Costa^2^7,76  Pereira-Silva, Jordam William^1^1,10,102 

[1] Aerodyne Research Inc, Billerica, MA 01821, USA^23;Atmospheric Turbulence and Diffusion Division, ARL, National Oceanic and Atmospheric Administration, Oak Ridge, USA^66;Beilun Bureau of Meteorology, Ningbo 315800, China^3;British Geological Survey, Environmental Science Centre, Keyworth, Nottingham NG12 5GG, UK^94;Brookhaven National Laboratory, Upton, NY, USA^18;CAS Center for Excellence in Regional Atmospheric Environment, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, 361000, Fujian, China^29;CASP, West Building, Madingley Rise, Madingley Road, CB3 0UD, Cambridge, UK^38;Centers for Disease Control and Prevention, Atlanta, USA^108;China Center for Disease Control and Prevention, Beijing, China^110;Cold Regions Research Centre, Wilfrid Laurier University, Waterloo, Ontario, Canada^52;Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters, Nanjing University of Information Science & Technology, Nanjing 210044, China^4;College of Pharmacy, Anhui Medical University, 81 Meishan Road, Hefei 230032, China^30;Czech University of Life Sciences, Faculty of Environmental Sciences, Prague, Czech Republic^44;Delegación Territorial de AEMET (Spanish National Meteorological Agency) en Aragon, Zaragoza, Spain^75;Department of Animal Ecology & Systematics, Justus Liebig University, Heinrich-Buff-Ring 26–32 IFZ, 35392 Giessen, Germany^35;Department of Atmospheric Sciences and Department of Chemistry, Texas A&M University, College Station, TX 77843, USA^21;Department of Chemistry and Cooperative Institute for Research in Environmental Sciences, University of Colorado, Boulder, CO, USA^9;Department of Civil and Environmental Engineering, Imperial College London, London, UK^63;Department of Civil and Environmental Engineering, University of California, Berkeley, Berkeley, CA, USA^12;Department of Civil and Environmental Engineering, University of Waterloo, Waterloo, Ontario, Canada^56;Department of Earth Sciences, Western University, London, Ontario, Canada^61;Department of Earth and Planetary Sciences, Harvard University, Cambridge, MA, USA^20;Department of Environment, Water and Natural Resources, Government of South Australia, Adelaide, SA 5000, Australia^101;Department of Environmental Science, Policy, and Management, University of California, Berkeley, CA, USA^11;Department of Environmental Sciences, Universidade Federal de São Paulo, Diadema, São Paulo, Brazil^8;Department of Geography, University of Guelph, Guelph, Ontario, Canada^53;Department of Geography, University of Innsbruck, Innsbruck, Austria^95;Department of Geography, University of Lethbridge, Lethbridge, Alberta, Canada^55;Department of Geology, Faculty of Geology and Geophysics, University of Bucharest, Bălcescu Bd. 1, 010041 Bucharest, Romania^37;Department of Geology, University of Namur, Rue de Bruxelles 61, 5000 Namur, Belgium^93;Department of Geoscience, University of Calgary, Calgary, Alberta, Canada^57;Department of Geosciences and Engineering, Delft University of Technology, Stevinweg 1, 2628 CN Delft, the Netherlands^41;Department of Geosciences, University of Oslo, P.O. Box 1022 Blindern, 0315 Oslo, Norway^84;Department of Hydrology and Atmospheric Sciences, University of Arizona, Tucson, AZ, USA^46;Department of Renewable Resources, University of Alberta, Edmonton, Alberta, Canada^58;Department of Statistics, University of Innsbruck, Innsbruck, Austria^106;Dipartimento di Ingegneria Civile, Ambientale e Architettura, Università di Cagliari, Cagliari, Italy^80;Dipartimento di Matematica e Informatica, Università di Cagliari, Cagliari, Italy^81;Division for Biomedical Physics, Medical University of Innsbruck, Innsbruck, Austria^105;Division of Geological and Planetary Sciences, California Institute of Technology, Pasadena, CA 91125, USA^25;Dép. Biologie, Chimie et Géographie, groupe BOREAS and Québec-Océan, Université du Québec à Rimouski, 300 allée des ursulines, Rimouski, Qc, G5L 3A1, Canada^88;Département de Géographie and Centre d'études nordiques, Université de Montréal, Montréal, Quebec, Canada^62;ENVEO IT GmbH, Innsbruck, Austria^98;EPOC, UMR 5805, Université de Bordeaux, Allée Geoffroy Saint-Hilaire, 33615 Pessac, France^85;Environment Research Institute, Shandong University, Qingdao 266237, China^24;Environment and Climate Change Canada, Saskatoon, Canada^73;Environment and Climate Change Canada, Toronto, Canada^67;Environment and Climate Change Canada, Victoria, Canada^74;Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA, USA^19;Environmental and Climate Sciences Department, Brookhaven National Laboratory, Upton, New York 11973-5000, USA^6;Faculty of Physics and Electrical Engineering, University of Bremen, Bremen, Germany^50;Fundação Oswaldo Cruz, Manaus, Brasil^123;Fundação Oswaldo Cruz-Fiocruz, Rio de Janeiro, Brasil^113;Fundação de Medicina Tropical Dr Heitor Vieira Dourado, Manaus, Brasil^115;GET-GRGS, UMR 5563, CNRS/IRD/UPS, Observatoire Midi-Pyrénées, 31400 Toulouse, France^86;GHD Canada, Waterloo, Ontario, Canada^54;Geology and Applied Geology Unit, Faculty of Engineering, University of Mons, Place du Parc 20, 7000 Mons, Belgium^89;Georgikon Faculty, University of Pannonia, Deák Ferenc u. 16, 8360 Keszthely, Hungary^48;Grantham Institute – Climate Change and the Environment, Imperial College London, London, UK^64;Grigore Antipa National Museum of Natural History, Sos. Kiseleff Nr. 1, 011341 Bucharest, Romania^39;Groundwater and Quaternary Geology Mapping Department, Geological Survey of Denmark and Greenland, Aarhus, Denmark^82;Guangzhou Key Laboratory Environmental Catalysis and Pollution Control, Guangdong Key Laboratory of Environmental Catalysis and Health Risk Control, School of Environmental Science and Engineering, Institute of Environmental Health and Pollution Control, Guangdong University of Technology, Guangzhou 510006, China^22;HR Wallingford, Howbery Park, Wallingford, Oxfordshire OX10 8BA, UK^65;Institute for Soil Sciences and Agricultural Chemistry, Centre for Agricultural Research, Hungarian Academy of Sciences, Herman Ottó út 15, 1022 Budapest, Hungary^47;Institute of Atmospheric and Cryospheric Sciences, University of Innsbruck, Innsbruck, Austria^103;Institute of Hydrology and Water Resources Management, Leibniz Universität Hannover, Hannover, Germany^97;Institute of Physics, University of São Paulo, São Paulo, Brazil^14;Institute of Urban Meteorology, China Meteorological Administration, Beijing 100089, China^2;Instituto Adolfo Lutz, São Paulo, Brasil^122;Integrated Water Systems and Governance, IHE Delft, Delft, the Netherlands^76;Invited contribution by Arnaud Watlet, recipient of the EGU Hydrological Sciences Outstanding Student Poster and PICO Award 2016^91;John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, USA^7;Kansas Geological Survey, University of Kansas, Lawrence, KS 66047, USA^102;Karst Research Institute ZRC SAZU, Titov trg 2, 6230 Postojna, Slovenia^51;Key Lab. of Environmental Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Hefei 230031, China^26;Key Laboratory for Aerosol-Cloud-Precipitation of China Meteorological Administration, School of Atmospheric Physics, Nanjing University of Information Science & Technology, Nanjing 210044, China^5;Korea Institute of Science and Technology, Seoul, 02792, Republic of Korea^100;LEGOS-GRGS, UMR 5566, CNRS/IRD/UPS, Observatoire Midi-Pyrénées, 31400 Toulouse, France^87;Laboratoire de Planétologie et Géodynamique – Nantes (LPGN), UFR Sciences et Techniques, Université de Nantes, UMR-CNRS 6112, Rue de la Houssinière 2, BP92208, 44322 Nantes CEDEX 3, France^92;LuftBlick, Innsbruck, Austria^104;Mediterranean Agronomic Institute (MAIB) – Land and Water Department, Valenzano (Bari), Italy^78;Meteorological Service of Canada, Environment and Climate Change Canada, Dartmouth, Canada^71;Meteoswiss, Payerne, Switzerland^72;Ministerio de Salud, Santiago, Chile^118;NIOZ Royal Netherlands Institute for Sea Research, Department of Ocean Systems, 1790 AB Den Burg, the Netherlands^40;Nacional Colonia La Campaña, Tegucigalpa, Honduras^111;National Center For Atmospheric Research, Research Application Laboratory, Boulder, CO, USA^42;National Center for Atmospheric Research, Boulder, USA^70;National Centre for Groundwater Research and Training, School of the Environment, Flinders University, Adelaide, SA 5001, Australia^99;National Hydrology Research Centre, Saskatoon, Saskatchewan, Canada^59;National Institute of Marine Geology and Geoecology (GeoEcoMar), 23–25 Dimitrie Onciul St., 024053 Bucharest, Romania^36;Naturalis Biodiversity Center, P.O. Box 9517, 2300 RA Leiden, the Netherlands^32;Norwegian Meteorological Institute, Oslo, Norway^69;Palaeomagnetic Laboratory “Fort Hoofddijk”, Faculty of Geosciences, Utrecht University, Budapestlaan 17, 3584 CD Utrecht, the Netherlands^34;Pan American Health Organization, Washington DC, USA^112;School of Environmental Science and Optoelectronic Technology, University of Science and Technology of China, Hefei 230027, China^28;School of Mathematics and Physics, Anhui University of Technology, Ma'anshan 243032, China^31;Science Island Branch of Graduate School, University of Science and Technology of China, Hefei 230026, China^27;Seismology-Gravimetry, Royal Observatory of Belgium, Avenue Circulaire 3, 1180 Uccle, Belgium^90;Southern Illinois University Carbondale, Carbondale, USA^120;Southern Illinois University, Carbondale, USA^119;State Environmental Protection Key Laboratory of Urban Ambient Air Particulate Matter Pollution Prevention and Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300071, China^1;State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, P.R. China^83;These authors contributed equally to this work^33;UFZ-Helmholtz Centre for Environmental Research, Department of Computational Hydrosystems, Leipzig, Germany^43;Universidad Metropolitana de Ciencias de la Educación, Santiago, Chile^117;Universidad de Chile, Santiago, Chile^116;Universidade de Brasília, Brasília, Brasil^107;Universidade do Estado do Amazonas, Manaus, Brasil^114;University of Basilicata, School of Agricultural, Forestry and Environmental Sciences – Hydraulics and Hydrology Division, Potenza, Italy^79;University of Ghana, Legon, Ghana^109;Water Institute and Department of Earth and Environmental Sciences, University of Waterloo, Waterloo, Ontario, Canada^60;Water Resources Section, Delft University of Technology, Delft, the Netherlands^77;Wegener Center for Climate and Global Change, University of Graz, Graz, Austria^96;Western Kentucky University, Bowling Green, USA^121;World Meteorological Organization, Geneva, Switzerland^68;now at: Agrarian Sciences Institute, Federal University of Uberlândia, Minas Gerais, Brazil^16;now at: Coldwater Laboratory, University of Saskatchewan, Canmore, Alberta, Canada^45;now at: College of Environmental Science and Engineering, Peking University, Beijing, China^17;now at: Department of Atmospheric Sciences, University of Washington, Seattle, WA, USA^10;now at: Department of Civil and Environmental Engineering, Virginia Tech, Blacksburg, VA, USA^13;now at: IMT Lille Douai, Université Lille, SAGE, Lille, France^15;previously published under the name Tóth^49

关键词: Zika virus;    sexual transmission;    mosquito vectors;    mating;    Flavivirus;   

DOI  :  10.1590/0074-02760170329

学科分类：地质学

来源: Bioline International

【 摘 要 】

BACKGROUND Aedes aegypti is considered the main Zika virus (ZIKV) vector, and is thought to be responsible for the 2015-2016 outbreak in Brazil. Zika positive Ae. aegypti males collected in the field suggest that vertical and/or venereal transmission of ZIKV may occur. OBJECTIVES In this study, we aimed to demonstrate that venereal transmission of ZIKV by Ae. aegypti can occur under laboratory conditions. METHODS Ae. aegypti collected in the city of Manaus, confirmed as negative for Zika, Dengue and Chikungunya virus by reverse transcription real-time polymerase chain reaction (RT-qPCR) (AaM3V- strain), were reared under laboratory conditions and used for the experiments. The ZIKV used in this study was isolated from a patient presenting with symptoms; ZIKV was confirmed by RT-qPCR. Experiment 1: virgin male mosquitoes of AaM3V- strain were intrathoracically inoculated with a ZIKV suspension; four days after injection, they were transferred to a cage containing virgin females of AaM3V- strain and left to copulate for five days. Experiment 2: virgin female mosquitoes of AaM3V- strain were orally infected with a ZIKV suspension by blood feeding membrane assay; nine days after blood feeding, they were placed in cages with Ae. aegypti AaM3V- virgin males and left to copulate for four days. After copulation, all mosquitoes were individually evaluated for viral infection by RT-qPCR. FINDINGS The mean infection rate in Experiment 1 and Experiment 2 was 45% and 35%, respectively. In both experiments, cycle threshold values ranged from 13 to 35, indicating the presence of viral genomes. MAIN CONCLUSION Ae. aegypti males intrathoracically inoculated with a ZIKV suspension are infected and can transmit the virus to uninfected females by mating. Moreover, Ae. aegypti females orally infected with a ZIKV suspension can transmit the virus to uninfected males by copulation. This study shows that ZIKV infection of Ae. aegypti mosquitoes occurs not only during blood feeding, but also during copulation.

【 授权许可】

Free