Delivery of Positively Charged Proteins Using Hyaluronic Acid Microgels

International Journal of Information Technology

Elaheh Jooybar ; Mohammad J. Abdekhodaie ; Marcel Karperien ; Pieter J. Dijkstra

关键词: Microgel;    inverse emulsion;    protein delivery;    hyaluronic acid;    crosslinking.;   

DOI  :  10.1999/1307-6892/10009787

学科分类：计算机应用

来源: World Academy of Science, Engineering and Technology (W A S E T)

【 摘 要 】

In this study, hyaluronic acid (HA) microgels were developed for the goal of protein delivery. First, a hyaluronic acid-tyramine conjugate (HA-TA) was synthesized with a degree of substitution of 13 TA moieties per 100 disaccharide units. Then, HA-TA microdroplets were produced using a water in oil emulsion method and crosslinked in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H2O2). Loading capacity and the release kinetics of lysozyme and BSA, as model proteins, were investigated. It was shown that lysozyme, a cationic protein, can be incorporated efficiently in the HA microgels, while the loading efficiency for BSA, as a negatively charged protein, is low. The release profile of lysozyme showed a sustained release over a period of one month. The results demonstrated that the HA-TA microgels are a good carrier for spatial delivery of cationic proteins for biomedical applications.

【 授权许可】

Unknown