Effect of 2, 6-Dichlorobenzonitrile on Amoebicidal Activity of Multipurpose Contact Lens Disinfecting Solutions

The Korean Journal of Parasitology

Fu-Shi Quan^1^3¹  Eun-Kyung Moon^1²  Seungeun Lee^2³ 

[1] Biomedical Science Institute, Kyung Hee University, Seoul 02447, Korea^3;Department of Medical Zoology, Kyung Hee University School of Medicine, Seoul 02447, Korea^1;Department of Parasitology, Dong-A University College of Medicine, Busan 49201, Korea^2

关键词: Acanthamoeba;    amoebicidal effect;    MPDS;    DCB;    cytotoxicity;   

DOI  :  10.3347/kjp.2018.56.5.491

学科分类：生物科学（综合）

来源: The Korean Society for Parasitology

【 摘 要 】

Multipurpose contact lens disinfecting solutions (MPDS) are widely used to cleanse and disinfect microorganisms. However, disinfection efficacy of these MPDS against Acanthamoeba cyst remain insufficient. 2, 6-dichlorobenzonitrile (DCB), a cellulose synthesis inhibitor, is capable of increasing the amoebical effect against Acanthamoeba by inhibiting its encystation. In this study, we investigated the possibility of DCB as a disinfecting agent to improve the amoebicidal activity of MPDS against Acanthamoeba cyst. Eight commercial MPDS (from a to h) were assessed, all of which displayed insufficient amoebicidal activity against the mature cysts. Solution e, f, and h showed strong amoebicidal effect on the immature cysts. Amoebicidal efficacy against mature cysts remained inadequate even when the 8 MPDS were combined with 100 μM DCB. However, 4 kinds of MPDS (solution d, e, f, and h) including 100 μM DCB demonstrated strong amoebicidal activity against the immature cysts. The amoebicidal activity of solution d was increased by addition of DCB. Cytotoxicity was absent in human corneal epithelial cells treated with either DCB or mixture of DCB with MPDS. These results suggested that DCB can enhance the amoebicical activity of MPDS against Acanthamoeba immature cyst in vitro .

【 授权许可】

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