Pinus Roxburghii essential oil anticancer activity and chemical composition evaluation

EXCLI Journal

Munawar Iqbal¹  Qaisar Manzoor²  Amit Kumar Tyagi³  Raja Adil Sarfraz⁴  Arfaa Sajid⁵ 

[1] Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M.D. Anderson Cancer Center, Houston, Texas, USA;Department of Chemistry, The University of Lahore, Lahore, Pakistan;Department of Chemistry, University of Agriculture, Faisalabad, Pakistan;Department of Chemistry, University of Punjab, Lahore, Pakistan;Government College Women University Faisalabad, Faisalabad, Pakistan

关键词: Pinus roxburghii;    anticancer agent;    essential oil;    NF-kappaB;    MTT assay;   

DOI  :  10.17179/excli2016-670

学科分类：过敏症与临床免疫学

来源: University of Mainz, University of Leipzig

【 摘 要 】

The present study was conducted to appraise the anticancer activity of Pinus roxburghii essential oil along with chemical composition evaluation. MTT assay revealed cytotoxicity induction in colon, leukemia, multiple myeloma, pancreatic, head and neck and lung cancer cells exposed to essential oil. Cancer cell death was also observed through live/dead cell viability assay and FACS analysis. Apoptosis induced by essential oil was confirmed by cleavage of PARP and caspase-3 that suppressed the colony-forming ability of tumor cells and 50 % inhibition occurred at a dose of 25 μg/mL. Moreover, essential oil inhibited the activation of inflammatory transcription factor NF-κB and inhibited expression of NF-κB regulated gene products linked to cell survival (survivin, c-FLIP, Bcl-2, Bcl-xL, c-Myc, c-IAP2), proliferation (Cyclin D1) and metastasis (MMP-9). P. roxburghii essential oil has considerable anticancer activity and could be used as anticancer agent, which needs further investigation to identify and purify the bioactive compounds followed by in vivo studies.

【 授权许可】

CC BY